Влияние реципрокального типа движения инструмента на провоспалительные нейромедиаторы

Количественная оценка влияния представителей двух различных систем обработки корневого канала одним файлом на вещество П (ВП) и кальцитонин-ген связанный пептид (КГСП), содержащиеся в периодонтальной связке (ПС) после проведения эндодонтической обработки. Для получения сорока образцов периодонтальной связки были использованы интактные премоляры, удаленные по ортодонтическим показаниям. До удаления, 20 из них были разделены поровну на две группы, в которых корневые каналы были обработаны с использованием одной из двух однофайловых систем: WaveOne или Reciproc. Десять премоляров были подготовлены с использованием ручных файлов и служили в качестве положительной контрольной группы. Оставшиеся 10 премоляров были удалены без эндодонтического лечения и служили отрицательной контрольной группой. Все образцы периодонтальной связки (ПС) были обработаны для определения содержания вещества П (ВП) и кальцитонин-ген связанного пептида (КГСП) методом радиоиммунного анализа.
Заключение. Содержание вещества П и КГСП в клетках периодонта выше в зубах, подготовленных с помощью WaveOne и с помощью ручных инструментов. Обработка с помощью Reciproc позволяет поддерживать содержание ВП и КГСП на уровне интактных зубов.

Aim To quantify the effect of two singlefile reciprocating root canal preparation systems on Substance P (SP) and Calcitonin gene-related peptide (CGRP) expression in healthy human periodontal ligament (PDL).
Methodology Forty PDL samples were obtained from healthy premolars where extraction was indicated for orthodontic reasons. Prior to extraction, 20 of these premolars were divided equally in two groups, and then, root canals were prepared using one of two different singlefile systems: WaveOne and Reciproc. Ten premolars were prepared with hand files and served as a positive control group. The remaining 10 premolars where extracted without treatment and served as a negative control group. All PDL samples were processed, and SP and CGRP were measured by radioimmunoassay.
Conclusion Substance P and CGRP expression in PDL cells increased when teeth were prepared with WaveOne as well as with hand instrumentation. Reciproc maintained SP and CGRP levels in line with the negative control group.

кальцитонин-ген связанный пептид человека, периодонтальная связка, провоспалительные нейромедиаторы, однофайловые системы инструментов, вещество П.

Calcitonin gene-related peptide, human periodontal ligament, neurogenic inflammation, singlefile reciprocating systems, Substance P.

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